Antigenic characterization of small, round-structured viruses by immune electron microscopy.

Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization.

Application of reverse transcription (RT)-PCR to detect small round-structured viruses (SRSVs) from fecal specimens of patients with gastroenteritis has been insensitive because of the tremendous sequence heterogeneity between strains. We have designed two RT-PCR primer sets (G-1 and G-2) based on the nucleotide sequence diversity in the RNA polymerase gene of SRSVs belonging to two distinct ge...

Small round structured viruses (SRSVs) and transmission electron microscopy

Use of solid-phase immune electron microscopy for classification of Norwalk-like viruses into six antigenic groups from 10 outbreaks of gastroenteritis in the United States.

Norwalk-like viruses observed in fecal specimens from 10 outbreaks of gastroenteritis investigated in the United States between 1987 and 1992 were analyzed by solid-phase immune electron microscopy. Outbreak virus strains were classified into six antigenic groups: the four types (UK1 to UK4) previously defined in the United Kingdom, Norwalk virus, and the Oklahoma agent that was newly defined i...

Molecular detection and epidemiology of small round-structured viruses in outbreaks of gastroenteritis in the Netherlands.

To study the epidemiology of small round-structured viruses (SRSV) in the Netherlands, all outbreaks of gastroenteritis that were reported to the Research Laboratory for Infectious Diseases, Department of Virology, National Institute of Public Health and the Environment (RIVM) in 1994 and 1995 were examined using electron microscopy (EM), single-round reverse transcription-polymerase chain reac...

Virus-like particle, 35 to 40 nm, associated with an institutional outbreak of acute gastroenteritis in adults.

In an outbreak of acute gastroenteritis in an institution for the mentally retarded at Otofuke, Hokkaido, Japan, virus-like particles were observed by electron microscopy in five of seven stool specimens from the patients. The particles had 10 rod-shaped and 10 round projections or capsomeres on the periphery, measured 35 to 40 nm in diameter, and had a buoyant density of 1.35 to 1.37 g/ml in c...